https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Next generation sequencing analysis reveals segmental patterns of microRNA expression in mouse epididymal epithelial cells https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26480 Wed 11 Apr 2018 13:21:00 AEST ]]> Characterisation of mouse epididymosomes reveals a complex profile of microRNAs and a potential mechanism for modification of the sperm epigenome https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30207 350 microRNAs (miRNAs), a developmentally important sRNA class, the majority (~60%) of which are also represented by the miRNA signature of spermatozoa. This includes >50 miRNAs that were found exclusively in epididymal sperm and epididymosomes, but not in the surrounding soma. We also documented substantial changes in the epididymosome miRNA cargo, including significant fold changes in almost half of the miRNAs along the length of the epididymis. Finally, we provide the first direct evidence for the transfer of several prominent miRNA species between mouse epididymosomes and spermatozoa to afford novel insight into a mechanism of intercellular communication by which the sRNA payload of sperm can be selectively modified during their post-testicular maturation.]]> Wed 11 Apr 2018 11:13:04 AEST ]]> The microRNA signature of mouse spermatozoa is substantially modified during epididymal maturation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26158 Sat 24 Mar 2018 07:35:27 AEDT ]]>